The experimental plan is drawn up. The new reagent you ordered is in solution and ready to go. You seeded your cells into dishes the day before, and they are growing as expected. All of your calculations are in order. Everything is in place and looks great. You set up and run your experiment without any issues or hiccups and collect your samples 48 hours later. You complete your downstream analyses, pull up the data on your screen, and your heart sinks. Your baseline values are well above average, obscuring…
Tech Notes & Training
6 Questions to Ask Your Pipette Calibration Service Provider
Pipettes need to be serviced on a routine basis (usually once every 6 or 12 months) to ensure optimal performance. However, not all calibration providers offer high quality service. Options you may be considering for your pipette calibration include: 1) sending your units to a third-party calibration company, 2) having a technician come to your lab, or 3) sending your pipettes to the original manufacturer. Ask these 6 important questions to your calibration provider when deciding who will perform this important service for your pipettes. Do you perform Preventative Maintenance…
ali-Q Pipetting Techniques and Tips
Aliquoting / Repeat Pipetting / Multidispensing with ali-Q is faster and easier than with standard pipet controllers using serological pipets. Once you’ve dialed in the volume you want to multidispense and aspirated the liquid you need, simply press and hold the purple aliquot button for the entire duration of each aliquot. When the LED blinks green and the motor turns ON at the end of the dispense, release the button. The ali-Q is now ready for your next press and hold. For the best accuracy and precision using ali-Q, performing…
Improving Performance of Micro-volume Pipetting
Any experienced pipetter knows that when pipetting, the smaller the dispense volume, the harder it is to achieve accurate and consistent results. Pipetting volumes below 2.0 µL can be frustratingly difficult; the smallest drop left behind, the slightest movement, or the quality of the individual tip can cause poor results. A pipette that fails calibration for one technician, may pass for another. Not all variables can be easily controlled, but here are some techniques and tips that can help improve performance!
CODE: 234567 (sample)
